Change in WLP007 Behavior (washed yeast)

Ingredients
1

Something odd I noticed about my WLP007 starter (washed)…

When I built my first starter the clumps were much large. After using that starter, washing it, then building another starter, the clumps are much smaller. It still drops pretty quick and compacts at the bottom.

The starter fermented quick too.

If the clumping is flocculation, then it is flocculating different now.

Should I not save this from the next batch, opt for a new vial & start saving again?

For those of you that have washed and reused WLP007 - do you see the same thing (smaller clumping) or is something different going on with mine?

2

if you are washing you are likely getting the least flocculent cells carrying over each iteration so it’s not too surprising that it’s getting less chunky.  If you want the chunky behavior start over with a fresh culture. Or you could let it ride and see if you can develop your own house yeast.

3

I quit washing after one try. If my recycled slurry is fresh I just pitch a couple hundred mils, if it’s a couple-few weeks old I make a starter with 100 mils