Storing Harvested Yeast/Slurry

I have read various articles/posts about storing harvested yeast/slurry (both frozen and refridgerated), and noticed some concern about fluctuating temperatures harming the viability of the yeast.  I know the temperature controller I have on my Beer Fridge (converted from an upright freezer) can fluctuate 4 degrees.  Articles that discussed freezing yeast (I don’t do this yet) say that a frost free freezer should not be used because the yeast wiil alternatly freeze and thaw, freeze and thaw.

To the point of this post.  I was talking with the head brewer at a local brewery (Cheers to Highland Brewing in Asheville, NC) and he said to store the slurry in an ice bath in the fridge.  This will keep refridgerated samples at a more constant temp.  This hit me as such an easy solution.  I’m sure many of the more expirienced brewers do this, but I thought I would throw it out there for us “newbies”.

I’m not sure if you could over come the pitfalls of a frost free freezer by freezing the sample in ice.

Storing the yeast in an ice bath in the refrigerator would minimize the temp swings but you would have to monitor the bath as the ice melted and continue to add ice over time. I haven’t experienced any obvious issues with storing yeast in the refrigerator. I guess if it’s not broke, don’t fix it. YMMV

the ice bath might be a good idea, but I’m too lazy for that.

If you keep the door to the fridge shut I can’t imagine that it swings all that much either.

I, like many of the guys here, just keep my slurry in mason jars in the back of the fridge and have never had a problem.

Don’t sweat it too much.

Brewing is a ROBUST process and yeast are resistant bugs (Fungi).

I’m not sure that freezing yeast even in glycerin solutuion has any advantages over refrigerating them whis is much easier