Culturing from Commercial Beer

Just an update…finished two steps, everything looks good. Completing a 1 liter step this morning (picture from 8 hours after the 1 liter step), but I’m thinking in the past my starters were 2 liters. Thoughts?

That starter looks good. If you aerate your wort well, the difference in cell count between a 1L starter and a 2L starter is approximately 90 minutes of propagation time.  The one area where a larger starter for five gallons makes sense is higher gravity brewing.  We need to pitch a larger cell count with high-gravity brewing due to the initial cell loss to osmotic pressure.

“90 minutes of propagation time”, can you elaborate?

This wasn’t on a stir plate and I shook it up. Not sure how well that is.

IIRC 90 min is one growth cycle