This starter owes me money…
Nice!
A ton of this hobby is applying common sense…it seems like a ton of folks are not in touch with that…
Alestateyall just motivated me to pull a couple slants of 2352 out and start propagating them for my next brewday. Got my mini flasks in the autoclave now.
Using Saccharomyces’ size rule of 4 times larger, I’ve got a couple 100ml pyrex erlenmyers to shake 25mls like it owes me money. (Step 1)
?
I think he means you are applying common sense
Oh. Sorry I misunderstood.
A guy that I used to work with often said, “Why is common sense so uncommon?”
He was usually drunk when he said it.
Leaving aside discussions of medians, means and other statistical errata, I always liked the George Carlin version:
I’m usually doing something stupid when I think of it 
I’d say!
First try at propagation from a slant. Two days ago I pulled a little bit from a slant and inoculated 20ml. This morning I pitched those to 200ml. I think its actually working. What an amazing hobby!
Welcome to a fascinating hobby within a fascinating hobby! This event is the start of a completely new brewing experience, one without limits placed on you by the major yeast propagators. Every culture in the world is available to brewers who can propagate from slant or a spec of lyophilized yeast shipped in a glass ampule.
And I owe it to you, buddy. Thanks!
Amazing. Great job! This fascinates me, but I know it is too time consuming for what I have available right now. I’ll live vicariously!
Yep, good job Jim! Sometime I’ll get into this.
Thanks guys. Its really not that tough or time consuming. Pretty fun to see it work though
Very cool.
Me too.
I’ve made slants, but still haven’t been brave enough to use them. Your success is encouraging!
Phil, you need to go for it! Propagating a culture from slant is easier than taking a liquid culture through the single cell isolation process. You will be good as long as you perform your initial inoculation aseptically into autoclaved wort within the sphere of your sterile zone. You need to determine how wide the sterile zone is around your alcohol lamp or Bunsen burner. If you feel heat, then you can be fairly certain that there is an updraft that prevents dust from settling in/on your culture.