-----Original Message-----
From: thcipriani@gmail.com [mailto:thcipriani@gmail.com]
Sent: Wednesday, January 18, 2012 6:40 PM
To: Customerservice
Subject: Question From Wyeast Labs website for I have general question.
QUESTION:
Hello,
I have a question that pertains to Wyeast 3068 and other of the ‘plastic’ yeasts that may produce sulfur. I know that these yeast have a propensity to produce sulfur; however, I don’t know what makes certain fermentation schemes produce a greater amount of sulfur.
One of my current theories is that older yeast cells have a greater propensity to produce sulfur. I recently pitched a (12°P) hefeweiss beer with 20 million cells/mL and that fermentation was very sulfury. I’m using that information along with a a paper I read from the MBAA recently (it was from 1999, IIRC) that said a beer with a higher pitching rate has a greater amount of older cells in the finished beer since the final cell volume of the finished beer was the same regardless of pitching rate.
Can you provide any info on the 3068 yeast and sulfur production. Also, can you confirm that these yeast are slightly more elongated than the typical round fat yeast cells when viewed under a microscope. Or is the slightly elongated appearance indicative of something else?
Thanks!
FROM:
Tyler Cipriani thcipriani@gmail.com 80501
On Fri, Jan 20, 2012 at 9:40 AM, Labservices Labservices@wyeastlab.com wrote:
Hey Tyler,
Sorry it took me so long to get back to you. We don’t have info on sulfur production specifically related to 3068. One thing we know is 3068 does not store well after fermentation. It will die off quickly. This coincides with your info on population dynamics with respect to older populations of yeast when using higher pitch rates. For this strain, it is advised to use low pitch rates around 6 million cells/ml to promote ester production, specifically isoamyl acetate. At the pitch rates you mentioned, this ester will be at low levels. Possibly so low that you will not get the banana or bubblegum aromas desired with this strain.
As for cell morphology, yes, it is very normal for the cells to be elongated. Also, this strain is a chain-former, so it is common to see large groups of attached cells.
Let me know if this answers your questions.
Jess Caudill
Brewer/Microbiologist
Wyeast Laboratories, Inc.
P.O. Box 146
Odell, OR 97044 USA
Phone: 541-354-1335 Fax: 541-354-3449
jess@wyeastlab.com
www.wyeastlab.com
From: Tyler Cipriani [mailto:thcipriani@gmail.com]
Sent: Friday, January 20, 2012 9:31 AM
To: Labservices
Subject: Re: Question From Wyeast Labs website for I have general question.
Hey Jess,
Quick followup question about yeast growth and ester formation. You stated that 6 million cells/ml would promote ester production; however, I’ve read some conflicting information about this.
During intra cellular ester formation, from my reading I’ve surmised, enzymatic reactions create esters using alcohol and an acid - typically, Acetyl CoA. While many authors tend to agree that increased biomass production (i.e. creation of cell walls) reduces the Acetyl CoA that is available for ester production and leads to reduced ester levels in the beer (Narziss 2005, Cone, Noonan 1996, Fix 1999) authors differ with respect to ester production and yeast growth. Fix (Fix 1999) writes that any, “increased activity on the acetyl CoA branch”, whatever that means, will increase ester production while other authors (Narziss 2005, Cone) state that increased yeast growth leads to a decrease in esters since more of the acetyl CoA is used for sterol synthesis.
Is there more consensus on this topic now? Overall I’m confused about it - hence pitching a hefe (that I normally pitch around 7x10^6 cells/ml ) with double that rate - I was trying to get an increase in esters; however, the excess sulfur production made this “experiment” inconclusive.
Thanks for all your help! I just re-read my email and realized that customers like me are likely a giant pain in the ass.
Thanks Again,
Tyler
Sources:
Cone - http://www.danstaryeast.com/library/yeast-growth
Fix - George J. Fix Ph.D, Principles of Brewing Science, Brewers Publications, Boulder CO, 1999
Narziss - Prof. Dr. agr. Ludwig Narziss, Prof. Dr.-Ing. habil. Werner Back, Technische Universitaet Muenchen (Fakultaet fuer Brauwesen, Weihenstephan), Abriss der Bierbrauerei. WILEY-VCH Verlags GmbH Weinheim Germany, 2005
Noonan - Gregory J. Noonan, New Brewing Lager Beer, Brewers Publications, Boulder CO, 1996
Tyler,
Yes, customers like you are a total pain in the as[sic]. Just kidding.
I am definitely aware of the articles and text that describes lower ester production with higher yeast growth. But, as you know, what actually happens in practice and what books say and experiments show can be completely different things. We ran trials using 3068 at pitch rates of 3, 6, 12 and 24 million cells per ml. The 3 and 6 had strong banana aromas, the 12 million had very slight banana aroma and 24 million and no banana, and for a completely non-technical description, tasted like crap. More specifically, it had a strong styrene aroma along with burnt aromas.
Once we conducted these trials, we helped a ton of breweries refine the flavor profile of beers made from this strain. Basically most of the breweries were pitching the correct amount on the initial pitch from us. Upon repitching, they were losing the banana aroma completely. Most were reptiching 2-3 times the pitch rate of the original. When they reduced the repitch volumes the banana came back.
Jess