Darn it. Now I wish I know where the things came from. I’m also convinced that it wasn’t a simple bacterium due to its size. At least it doesn’t seem to be in my beer.
Babalu, to count yeast cells you’ll need a counting chamber (a.k.a. hemacytometer (sp?)) Otherwise you can’t correlate the count to the volume.
This showed me that I really have to work on finding a low cost way of taking microscope pictures.
Most of us already have digital cameras and all that is needed to take pictures with the microscope is an adapter. Depending on the quality these seem to cost between ~$40 and $200+.
I have not tried it out. I have not been impressed with any photos from low cost cameras. My last light microscope setup was a Zeiss, and it was a comfortable 5 figures. I expect that this product is a cmos chip on a flimsy digital setup with an adapter to fit an eyepiece, but you are pointing out the need to be able to photograph something. I do not expect that you would be able to take publication quality photos with any $59 camera, whether it is attatched to a microscope or not. If you want to show a picture of something to a microbiologist or folks on the list and ask them “what the %$#! is this?” I suspect it will do the trick.
For a camera, you might try cannibalizing a web cam. I did this ~10 yrs ago and it worked well enough. I can’t remember the details unfortunately, but it did work
Tonight I just took a Point&Shoot camera and pushed its lens onto the eye piece. It worked surprisingly well:
These are cells from a WLP833 yeast starter at 400x on a hemacytomter. I didn’t take a pic of the small grid which I use for counting since I wanted to include a non-viable cell. That cell stained blue with methylene blue. Almost all of the cells in the sample were alive and I had to search for a dead one.
While the quality works for posts here it is not as nice as I want it. Especially the outer regions are blurry.
No “things” in this sample though.
Correction: the width between the two grid lines shown is 0.25 mm and not 0.2 mm.