I’m looking for advice on a 2- or 3-stage yeast starter. I’m thinking of the following method, so would some of you more experienced folks lend a hand with a confirmation and/or advice, please?
Here’s what I’m planning:
If I do a 1.5L starter, assuming 100B starting yeast cells, I should finish with 181B cells (i.e. 1.8x multiplier). If I split the starter into 2 flasks, each being a 2nd stage (using chill & decant), wouldn’t I end up with (0.9B * 1.8) * 2 = 3.24B cells?
If you don’t do an actual cell count you’ll never know. The most important thing is that you pitch viable yeast, and that your cell count is in the ball park. There are a number of calculators out there that will do this for you.
Instead of splitting the starter into separate flasks, you have the option of decanting the 1st starter and add fresh wort on top of the existing yeast cake in you Erlenmeyer flask.
Assume 100-150 billion new cells per liter of ~1.030 wort. If you want to end up with ~320B cells total, put the pack in a 2 L stirred starter and if anything you’ll probably be overpitching a bit.