I’ve been kinda busy. I’ve had a yeast starter (Wyeast 1056) going for over a week now (1L size, 1.040 OG). Nice and creamy yellow color, still got a small dimple in the top of the fluid.
Assumming the yeast are done eating all the sugars and have attained maximum growth and reproduction, am I harming the yeast by keeping it on the stir plate and aerating it? (Air only, no Oxygen).
If you’re not pitching at high krausen, you’ll want to refrigerate it once it ferments out. It’s not so much the air, but the warm temperature and waste products that could lead to decreased viability over time.
As an aside and somewhat along the same lines I have killed a yeast starter with pure o2 before. But it would be impossible to do this with pumped air or on a stir plate.